Protein processing map of poliovirus
نویسندگان
چکیده
منابع مشابه
Poliovirus protein 3CD is the active protease for processing of the precursor protein P1 in vitro.
A transcription/translation system for generating poliovirus proteins in vitro has been used to assess the proteolytic activity of various polypeptides containing the virus-coded 3C region towards the poliovirus precursor protein P1. Plasmids containing a phage T7 promoter followed by either the complete poliovirus P1 sequence or various sequences containing the 3C region were used for this pur...
متن کاملA protein linkage map of the P2 nonstructural proteins of poliovirus.
The yeast two-hybrid system was used to catalog all detectable interactions among the P2 nonstructural cleavage products of poliovirus type 1 (Mahoney). Evidence has been obtained for specific associations among 2A(pro), 2BC, 2C, and 2B. Specifically, 2A(pro) can interact with itself and 2BC and its cleavage products (2B and 2C) interact in all possible combinations, with the exception of 2C/2C...
متن کاملPoliovirus-mediated disruption of cytoplasmic processing bodies.
Metazoan cells form cytoplasmic mRNA granules such as stress granules (SG) and processing bodies (P bodies) that are proposed to be sites of aggregated, translationally silenced mRNAs and mRNA degradation. Poliovirus (PV) is a plus-strand RNA virus containing a genome that is a functional mRNA; thus, we investigated if PV antagonizes the processes that lead to formation of these structures. We ...
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The NorFor system is a semi-mechanistic, which predicts nutrient supply and requirements for maintenance, milk production, growth and pregnancy in catlle. This research is aimed to evaluate the effects of grinding, pelleting and steam-flaking of corn grain on protein characteristic using NorFor model was tested according to completely randomized design. Degradability of dry matter and protei...
متن کاملCell-free synthesis and processing of the proteins of poliovirus.
Poliovirus RNA can be translated completely and accurately in rabbit reticulocyte lysates; the nascent poly-protein is processed to give primary products 1a, X, and 1b indistinguishable from those made in poliovirus-infected HeLa cells. The capsid precursor protein 1a is processed to form the capsid proteins VP0, VP1, and VP3, while the noncapsid precursor 1b is processed to form protein 2.
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1984
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.49.3.873-880.1984